Duruflé was born in Louviers in Normandy, France. At the age of ten, he entered the choir school at the Rouen cathedral, where he studied piano, organ, and theory with Jules Haelling. It is during this time that Duruflé developed his affinity for Gregorian chant. He moved to Paris in 1919 and studied with Charles Tournemire, organist at St. Clotilde, where Duruflé later became his assistant. He also later became the assistant of Louis Vierne at Notre Dame. In 1920, Duruflé entered the Paris Conservatoire and studied organ with Eugène Gigout, harmony with Jean Gallon, accompaniment with Estyle, counterpoint and fugue with Georges Caussade, and composition with Paul Dukas. At the Conservatoire, Duruflé took first prizes in the areas of organ, harmony, accompaniment, counterpoint and fugue, and composition. In 1929, he won a prize from the Amis de l'Orgue for interpretation and improvisation.
In 1930, he was appointed to the position of organist at St. Etienne-du-Mont, which he held for the remainder of his life, sharing the appointment with his wife after 1953. That same year, he was again honored by the Amis de l'Orgue, this time for his Prelude, adagio et choral varié sur le "Veni Creator," Op. 4. In 1936, Duruflé received the Blumenthal Foundation Prize. He became Dupré's assistant for the organ class at the Paris Conservatoire in 1943. He was also appointed professor of harmony, a position that he held until 1969. The Duruflé Requiem was premiered in 1947 by Desormière. In addition, Duruflé was a highly esteemed organist, and he toured extensively throughout Europe and North America. His performing career was ended by an automobile accident in May 1975 that left him virtually bedridden until his death in 1986. ~ Stephen Kingsbury, Rovi